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Before (time point: 0 h) and after incubation for 12, 20 and 40 h at 24 C whilst shaking (150 rpm), aliquots (2.0 mL) were taken, autoclaved (15 min, 121 C), pellets collected by centrifugation (13000 rpm, 4 min) and extracted with EtOH (1 mL) by shaking overnight (15 h) at room temperature

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