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To separate the entrapped and unentrapped drugs, the produced formulation was sonicated for 30 min before being centrifuged for one hour at 15,000 rpm and 4 C in a cooling centrifuge

The following themes summarize key areas of ongoing investigation: N NMT, NAD , and Energy Metabolism NNMT catalyzes the methylation of nicotinamide, diverting potential NAD precursors into alternative metabolites

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