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In-gel fluorescence assay In 200L PCR tubes, 0.1% DMSO, LCS3, or C1-27 was added to 0.5g of recombinant GSTO1 (Sigma, GS75-100ug) in PBS in 12uL total volume and incubated in a thermocycler at 37C for 20 mins in a competitive CMFDA binding assay described previously (28)