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In between runs, samples were cooled by incubating them on ice for 5 min

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Recent research has highlighted the potential for microbiome-based therapeutic strategies, such as probiotics, prebiotics, fecal microbiota transplantation (FMT), and dietary modifications, to restore microbial balance and alleviate disease symptoms

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Future research directions may include the development of targeted drugs based on recently identified molecular targets such as AGEs, SHBG, and miRNAs, which show promising therapeutic potential