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DSIP is primarily used to study signaling pathways associated with the hypothalamicpituitaryadrenal axis, circadian rhythm regulation, and neuropeptide-mediated communication

Briefly, muscles were collected, trimmed of connective tissue and fat, minced with scissors, then digested with 1.25 mg/ml of protease type XIV for 1 h at 37C

This might explain the recent focus on discussing ways to reduce heart disease risk in autoimmune patients