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Therefore, the differences in these proteins could explain the reduced expression of GshF in the mutant ZJ12345 compared to the wild type strain and help explain why the mutant required a higher IPTG concentration to obtain GshF activity similar to the wild type host strain

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This was followed by transduction with iCas9 and guide expressing vectors (Control and sg Gstt1 ), harvesting by trypsinization, washing twice in PBS, resuspension at 1 x 10 4 in 90ul PBS and 10% Matrigel (Corning), and injected into the 4 th inguinal mammary fat pad of BALB/c mice (Jackson Laboratory) to establish mammary tumors

Consequently, long-term CM may contribute to a healthier intestinal microbiota, potentially improving gut-brain communication through multiple mechanisms, including microbiota regulation (335)

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