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191,192 Following the activation of GLP-1R, the subunits of GPCRs can directly activate Class I PI3Ks
pmid:17090532 18
Briefly, fibroblast cells grown on a six-well plate were fixed in 1X fixative solution containing 2% formaldehyde and 2% glutaraldehyde for 10 minutes and subsequently stained overnight (15 hours) at 37 C with the -galactosidase staining solution at pH 6.0 for 15 hours
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