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The newly developed ferroptosis inducing compound ferroptocide is a Trx inhibitor [96]

H 2 O 2 oxidizes cysteine residues (Cys257, Cys268, and Cys568) on RIPK1, a process facilitating RIPK3 recruitment to the necrosome 70

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We expressed this in a eukaryotic expression system because this is the only established system that is suitable for the expression of complex recombinant proteins with human-like glycoforms that are bioactive in humans (Maccani et al