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The mice were maintained on the same diet used to induce DIO by Jackson Lab, 60 kcal% from high-fat diet (D12492i, Research Diets Inc., New Brunswick, NJ, USA) with ad libitum access and sterile water was provided from the Lixit System

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Research on HGF/c-Met signaling in other biological contexts demonstrates receptor internalization beginning around 4872 hours of sustained activation, with maximal downregulation occurring by day 57 of continuous agonist exposure