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After incubation on ice for 1 h, the homogenates were centrifuged at 20,400 g for 20 min at 4 C, and the resulting supernatants were appropriately diluted and used in the assays

If the protocol concerns you or conflicts with your current injection schedule, raise it with your prescribing physician before starting

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1) XJB-5-131 (mitochondrial nitroxide) inhibits lipid peroxidation to prevent ferroptosis, reducing inflammatory infiltration and promoting tubular repair (113)

However, if it was exposed to significant heat, its safer to discard it to ensure data integrity