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Using RNA-seq and real-time qPCR approaches, we demonstrated enrichment of PPARA in BWS lines relative to control lines, as differentiation proceeded

One microgram of total RNA from each sample was reverse transcribed, and the real-time RT-PCR measurements were performed, as described previously [49], using an apparatus Aria MX (Agilent Technologies, Santa Clara, CA, USA) with the corresponding SYBR Green kit, according to the Promega manufacturers recommendations

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D) An expanded view of aerobic NO scavenging demonstrates the subtle effects of AA and DHA