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Cells treated with test compounds were washed with PBS and incubated with 10 M 2,7-dichlorofluorescein diacetate (DCFH-DA) at 37 C for 30 min, followed by washing with PBS, digestion with trypsin, and collection

The resulted proteinGroups.txt file was filtered using R scripts, and proteins labeled as Potential contaminant, Reverse or Only identified by site were discarded

We recommend the 1ml calculation for its simplicity

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