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This design preserves long-term plasma exposure and steady-state signaling thresholds without requiring continuous infusion protocols

It has two completely independent domains

The M1 phenotype, stimulated by lipopolysaccharide (LPS) and interferon- (IFN-), was used as a positive control, whereas the M2 phenotype, induced by interleukin-4 (IL-4), functioned as a negative control

229 Although evolved to sense metals or metal-complexes directly, the set-point or sensitivity of these riboswitches to bioavailable metal may not fully align with the set-point of the analogous protein-based sensors given the complexity of the regulatory response involved (Section 3.3)

Chronic diabetic condition promotes impaired compensatory response to the antioxidant system or increased generation of free radicals thus, diabetic subjects may have a compromised antioxidant defense system

Mouse metabolism, mouse fat biology, and mouse dosing all differ from the human versions, and metabolic findings in rodents have a long history of not carrying over cleanly to people