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The relative signal intensity of the (CH) band at 800 cm 1 increased as a function of the treatment time in the presence of complex 2

If you tried that with plain sterile water, the risk of contamination and bacterial growth in the vial after the first use would be high, since theres nothing to prevent bacteria from growing once its opened

doi: 10.1007/s40618-015-0312-9 17 CrinADi GiorgioGLivieriCGrugniGBeccariaLBosioLet al

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While they act via identical mechanisms, AOD9604 is often preferred in oral administration studies due to its resistance to digestive enzymes, whereas standard 176-191 requires parenteral administration in research settings