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INS-1 cells were grown in RPMI containing 10% (vol/vol) fetal calf serum, 10 mM HEPES, 2 mM L-glutamine, 1 mM sodium pyruvate, and 0.05 mM 2-mercaptoethanol at 37 C with 5% CO 2
PMCID: PMC3257747
The rationale for combining L-arginine, apart from the mutually potentiating action, is the close pharmacokinetic profile, with both reaching the plasma peak between 30 and 60 min, and decrease in 1 to 2 hours
Proteins are more fragile than most people realize
Genetic variation in the GLP1R gene (rs6923761), GIPR receptor (rs1800437), and metabolic genes like FTO (rs9939609) influence both efficacy and side-effect tolerance
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