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M., Sajjakulnukit, P., Li, S., Crespo, J., Nwosu, Z

Timing considerations Most protocols recommend evening administration, 30-60 minutes before intended sleep

The big takeaway

Ct values obtained from RT-qPCR results performed with primers with primer activities between 1.9 and 2.2 were normalized using the 2-Ct method according to Livak and Schmittengen 73 criteria with the Ct value obtained for the specified reference gene (housekeeping)

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doi:10.1111/dom.13172