Induction of phase II detoxifying enzymes Many isothiocyanates are potent inducers of phase II detoxifying enzymes, including GSTs, UDP-glucuronosyl transferases (UGTs), NQO1, and glutamate cysteine ligase (GCL), that protect cells from DNA damage by carcinogens and reactive oxygen species (ROS) (25)
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Open 7 , e2350408e2350408 (2024)
The barcode fragment and pKS1 were both digested with Nco I and Bam HI and ligated with T4 DNA ligase (NEB) at a 5:1 (insert:vector) ratio (100 ng total DNA per 20 L reaction) overnight at 16 C
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Nutrients, 17(2), 345
