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After dehydration, the tissue was cleared in xylene for 15 min, followed by infiltration with soft and hard paraffin wax, and finally embedded in paraffin

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The role of short-chain fatty acids from gut microbiota in gut-brain communication

In the withdrawal trial, continuing preserved loss far better than switching to placebo, which regained about 14% over a year. The metabolic benefit fades in proportion to the weight regained, so a taper plus protein and resistance training beats a hard stop

Antonini S, Birtolo MF, Lania A, Longhi EV