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However, recent advancements have led to the development of promising therapeutic strategies, some of which have reached clinical trials or received FDA approval

Resulting protein solutions were subjected to filter-aided sample preparation (FASP) as described elsewhere 122 covering proteins alkylation (100 mM iodoacetamide (IAA) for 20 min in dark at laboratory temperature) and proteins digestion using 0.75 g of trypsin (sequencing grade

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Our results thus indicated that AITC treatment led to glutathione depletion which contributed to the higher susceptibility of glutathione mutants, but the molecular mechanism behind this physiological response is likely more complicated

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