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For long-term storage beyond one year, or for vials from a multi-month supply that will not be opened for extended periods, the freezer at -20 degrees Celsius is the better choice, extending stability to two years or more

HALO-Tag ligands were purchased from Promega

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The short answerit can vary by individual, but many people begin feeling noticeable effects within hours or days

The majority involve the lateral ligament complex, particularly the anterior talofibular ligament (ATFL), which sustains damage in approximately 85% of inversion injuries

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