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These collective findings demonstrate that VPA exposure induces systemic oxidative stress, characterized by concurrent antioxidant depletion (CAT, GSH-Px, GSH, SOD) and elevation of oxidative damage markers (MDA, T-NOS, NO)

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For the GSSCys assays, either GSH was varied between 50 M and 2.0 mM at fixed GSSCys concentrations (25, 50, 100 and 150 M) or GSSCys was varied between 25 and 200 M at fixed concentrations of GSH (0.05, 0.1, 0.2, 0.3 and 1.0 mM for ScGrx7 or 0.3, 0.5, 1.0 and 2.0 mM for PfGrx)