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Finally, the gels were washed twice in Milli-Q water

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Endocrinology 151:14731486 Hegedus L, Moses AC, Zdravkovic M, Le Thi T, Daniels GH (2001) GLP-1 and calcitonin concentration in humans: lack of evidence of calcitonin release from sequential screening in over 5000 subjects with type 2 diabetes or nondiabetic obese subjects treated with the human GLP-1 analog, liraglutide

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All measurements were taken following the guidelines provided by the manufacturer