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Cells were seeded on Falcon dishes at 37 C with 5% CO 2 in Dulbeccos modified Eagle medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin (Beyotime, China) and 100 mg/ml streptomycin (Beyotime, China) [26]

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Second, cystine is the primary imported form of cysteine 27 , but both cystine and cysteine are challenging to use due to rapid oxidation and insolubility 48

Progesterone helps support calming signals in the brain that promote stable sleep

investigators conducted clinician-rated assessments