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After centrifugation, protein extracts were collected and 50 g of protein lysates were resolved by SDS-PAGE and electrophoretically transferred onto Immobilon-P membranes (0.22 M

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Overall, microbially produced metabolites act as potent CNS regulators, either by modulating the vagus signaling and systemic immunity or by directly crossing the BBB (Deng et al., 2024)

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