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Typically, cells were seeded in 100 L RPMI phenol red free media per well in duplicates on a black 96-well plate with clear bottom (Thermo Scientific)
To euthanize the animals, the rats were anesthetized by putting them in a closed container with a piece of cotton saturated with an anesthetic agent (chloroform)
The liver metabolizes IS into indole, which raises the likelihood of peripheral vascular disease and vascular access thrombosis (6163)
Results do not disappear during off-cycles IGF-1 gradually returns to baseline over 2-4 weeks but the enhanced GH secretion depends on continued use
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