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van Minnen LP, Timmerman HM, Lutgendorff F, Verheem A, Harmsen W, Konstantinov SR, et al

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GLP1 medications are designed for subcutaneous injection (into the fat layer beneath the skin), not intramuscular injection

Clones were selected and grown in media lacking kanamycin, plated in media containing 2.5% galactose to select for loss of the plasmid, and screened by PCR for gene deletion

Confusion has arisen because the original CJC-1295 from ConjuChem Technologies contained DAC, yet other manufacturers subsequently began producing CJC-1295 without DAC