If you're new to injections, finding the right spot can feel a little overwhelming
*Correspondence: Marcella Reale, [email protected] This article was submitted to Non-Neuronal Cells, a section of the journal Frontiers in Cellular Neuroscience Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers
Additionally, subsequent studies should include additional negative controls for microbiota library preparation (e.g
DSIP, if effective, would layer on deeper slow-wave support rather than replace these staples
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For H 2 O 2 treatment, 100 M H 2 O 2 was added to RPMI 1640 growth medium (#1003461059, Sigma Aldrich, United Kingdom), without FBS and phenol red, supplemented with 15 mM HEPES (#1003098482, Sigma Aldrich, USA), 27.9 mM sodium bicarbonate (#101920550, Sigma Aldrich, Japan), 0.2 mM sodium pyruvate (#1002136564, Sigma Aldrich, Japan), 1 U/mL penicillin, and 1 g/mL streptomycin
