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A 1mg dose at 10mg/ml concentration = 0.1ml = 10 units A 2mg dose at 10mg/ml concentration = 0.2ml = 20 units A 3mg dose at 10mg/ml concentration = 0.3ml = 30 units At 20mg/ml concentration, unit-to-dose relationships double: 10 units = 0.1ml = 2mg peptide 5 units = 0.05ml = 1mg peptide Always calculate these relationships for your specific concentration before drawing doses

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2016;215:245.e1245.e7

In this approach, biological samples are reacted with HPE-IAM to alkylate endogenous supersulfides, and known amounts of isotope-labeled standards are then spiked into the same samples

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