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The Ca 2+ channel blockers -agatoxin and isradipine, respectively blocking voltage-gated Ca 2+ channels sensitive to -agatoxin (P/Q-type Ca 2+ channels) and L-type Ca 2+ channels, were from Alomone Labs (Jerusalem, Israel)

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Cell culture and generation of stable cell lines PathHunter CHO-GLP-1R -arrestin-1/-2 reporter cell lines (DiscoverX) were maintained in the manufacturers Culture Medium