BMC Syst Biol 2018;12:89
This activation upregulates both the expression and enzymatic activity of MMP-9, leading to degradation of critical structural components in the basement membrane of the blood-brain barrierparticularly type IV collagenthereby compromising barrier integrity and increasing permeability (105, 106)
These enzymes are called radical SAMs
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The assay measures the increase in absorbance monitored at 340 nm associated with the formation of NADPH, which is proportional to the aconitase activity
Bedir E, Karakoyun , Doan G, Kuru G, Kksolak M, Yusufolu H (2021) New cardenolides from biotransformation of gitoxigenin by the endophytic fungus alternaria eureka 1e1bl1: characterization and cytotoxic activities