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Sperm samples were centrifuged through a PureSperm 80/40 (Nidacon, Gothenburg, Sweden) gradient at at 1,500 rpm for 8 min

64 The sensitivity of L1 and L4/L5 lumbar axial deep tissues, evaluated by painrelated vocalization upon spinal compression using an algometer, was elevated in IVD degeneration animals compared to sham and agematched controls

Moreover, the PI3K/AKT/mTOR pathway can facilitate the expression of lipogenic genes in an SREBP-dependent manner (192), and mTORC1 has been regarded as a vital effector in advancing the trafficking or processing of SREBP to stimulate de novo lipogenesis (193)

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