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Such cross-contamination or additional stress to the sample may occur by applying too much pressure during tissue infiltration or due to microscope properties (strong light source, high temperature, lack of oxygen, water stress), but likewise during organelle isolation or fractionation (Zechmann 2014)

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The enhanced cocktails, CILy (CHIR+IDE1+Ly) and CIP (CHIR+IDE1+PD), significantly increased the expression of DE markers SOX17 and FOXA2, with CIP showing the highest efficiency, comparable to the traditional CA method