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Each test subject received an injection of 70 nmol of scopolamine hydrobromide in 2 ul of artificial cerebrospinal fluid that was delivered over a 20 second time period, 20 minutes prior to the water maze testing

The supernatants were collected and stored at 80 C for TAC content analysis, according to the method described by 53

Simons 2000

Media was removed and cells were fixed with methanol

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