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After labeling, cells were gently quenched, washed three times with DPBS, fixed and permeabilized with ice-cold methanol for 10 min

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Further, we showed that deficiency of UCP2 in mouse mast cells decreased the release of inflammatory mediators such as IL-6, PGD 2 , and histamine with inhibition of MAPKs activation (Tagen et al., 2009)

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