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More importantly, a large-scale 3b-phase randomized controlled trial (SURMOUNT-5), which compared semaglutide with tirzepatide in 751 obese adults without type 2 diabetes, explicitly reported that male patients had an average weight loss approximately 6 percentage points lower than female patients after 72 weeks of semaglutide treatment, further verifying that sex is an independent factor affecting semaglutides efficacy [80]

treated cells were incubated with JC-1 dye (5 g/mL) for 30 min at 37 C, followed by fluorescence detection using a fluorescence microscope, where the red/green fluorescence ratio was calculated to quantify mitochondrial polarization status, as a higher ratio indicates healthier mitochondrial function while a lower ratio suggests mitochondrial depolarization and dysfunction

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