RSMC and its mutant were induced by 0.02 mM IPTG at 37 C for 3 h, and GST-Sororin/GST-Wapl was induced by 0.04 mM IPTG at 25 C for 3 h
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We used an regression approach to pre-adjust for peptide, similar to what we did previously for phosphorylation data 81 : where is the abundance level for PTM j for individual i , is the intercept, peptide[ ij ] is the contribution of the corresponding unmodified peptide for PTM j for individual i , and i is the error for individual i , modeled as i N(0, 2 )
Its inactivation impairs MQC, promotes the accumulation of dysfunctional mitochondria, and accelerates dopaminergic neuronal loss
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