This overall design gives the impression of an enzyme that was built by retrofitting a new C-terminal active site onto an existing NADPH-dependent reductase (Schmidt and Davies, 2007), either by classical exon shuffling (Margulies and McCluskey, 1985) or by mutation of the stop codon and translational read-through into previously 3-untranslated sequences (Novoselov and Gladyshev, 2003)
In rat tendon studies, administration accelerated outgrowth, fibroblast migration, and post-injury cellular survival (PMID 21030672)
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Cells were then washed and stained with LIVE/DEAD Fixable Aqua Dead Cell Stain (Invitrogen, 1:1000) for 30 min
