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and use freezing (around 20 C) for longer-term preservation

Potential risks may include immunogenicity or impurities

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However, it is also possible that melanoma precursor cells originate from mature melanocytes in the basal cell layer of the epithelium that have undergone dedifferentiation following cytogenetic alterations or from immature melanocytes/naevomelanocytes arrested in the submucosa during migration from the neural crest which for reasons unknown have acquired a malignant phenotype [15]

Fromm GA, Lascano EF, Bur GE, Escalenta D

This education-driven model helps patients maintain results for years to come