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Even indirect sunlight over a few hours can initiate photodegradation

The membrane was then incubated at room temperature in blocking solution for 1 hour, followed by incubation in blocking solution containing the appropriate dilution of either phospho-Jak2 or the primary antibody for growth hormone receptor, for 2 hours

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Such a decay rate arises from a first-order reaction where the rate of elimination is proportional to the amount of the substance: [41] The half-life for this process is [41] Alternatively, half-life is given by where z is the slope of the terminal phase of the timeconcentration curve for the substance on a semilogarithmic scale