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Tissues were homogenized in RIPA buffer (Tris (50 mM), NaCl (150 mM), Triton X-100 (1%), disodium EDTA (1 mM), SDS (0.1%), sodium deoxycholate (0.5%)) containing protease and phosphatase inhibitors (S8830, SigmaFast, Thermo Scientific) followed with mild sonication (5 min, cycle: 30 s on/off

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As a GIP receptor agonist, tirzepatide enhances insulin secretion and may improve insulin sensitivity

Irritable Bowel Syndrome (IBS) Irritable Bowel Syndrome (IBS) Last Section Update: 10/2024 Contributor(s) : Shayna Sandhaus, PhD