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How the Dose Is Gradually Increased Over Time Retatrutide was escalated stepwise from 2 mg to up to 12 mg once weekly over approximately 12 weeks or longer, depending on individual tolerability and trial protocol

MCF-7 cell lines (ATCC, USA) were cultured in RPMI 1640 media (EuroClone S.p.A., Italy) supplemented with 1% penicillin-streptomycin, 1% L-Glutamine, and 10% fetal bovine serum (FBS) and incubated in a 37 C incubator under 5% CO2 atmosphere