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(2020) discovered that STIP1 homology and U-box containing protein 1 is the E3 ligase most likely to participate in IRP2 ubiquitin, but whether another significant ubiquitinase of IRP2, F-box and Leucine Rich Repeat Protein 5, is involved in this process has not been answered positively by the researchers and needs further investigation

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For purification, a 5 ml StrepTrap HP (GE Healthcare) was equilibrated with at least five column volumes of lysis buffer

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