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Many commenters recommended a delayed start date to ensure readiness and minimize technical and administrative disruptions to clinicians who will be required to build infrastructure to train staff, support clinical workflows, and submit data using eCQMs

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Co-Immunoprecipitation and Immunoblotting Analysis Cells were washed twice with phosphate-buffered saline (PBS), and then lysed in a lysis buffer on ice for 30 min, and then cell lysates were centrifuged at 12,500 rpm for 15 min

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