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Ultrathin sections obtained with an Ultracut EMFCS ultramicrotome (Leica Microsystems, Wetzlar, Germany) were examined unstained or poststained with uranyl acetate and lead hydroxide under a Morgagni 268D transmission electron microscope (FEI, Hillsboro, OR, USA) equipped with a Mega View II charge-coupled device camera (SIS, Soft Imaging System GmbH, Munster, Germany)