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Assuming the bands appear at the expected positions corresponding to their theoretical molecular weights, this indicates that the fusion proteins were expressed at the correct size and were not significantly degraded

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Cell culture HepaRG cells (Invitrogen, California, US) were cultured in growth medium (Williams E medium with Glutamax I, supplemented with 100 U/ml penicillin-streptomycin, 5 g/ml human recombinant insulin and 0.5 M hydrocortisone hemisuccinate) at 37 C with 5% CO2

The architecture of respiratory complex I