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Reconstitution B: 10 mg vial + 3 mL BAC water (3.33 mg/mL) more BAC water for larger, easier-to-measure draws

Add 1,000 L (1 mL) of reconstitution solvent to the 1 mg vial This yields a 1 mg/mL (1,000 g/mL) stock solution To achieve 100 g/mL working stock: Dilute 100 L of 1 mg/mL stock into 900 L of PBS or cell culture medium yields 100 g/mL in 1 mL To achieve 10 ng/mL in a 5 mL experiment: Take 0.5 L of 100 g/mL working stock + 4,999.5 L of medium Alternatively: dilute to an intermediate concentration to reduce pipetting error Use a dilution series approach (stock working stock final experimental concentration) to minimize pipetting inaccuracy at nanogram-level concentrations

Which nutrient is important for healthy skin and hair

doi: 10.1016/j.cell.2021.07.021 151 GarchowBMaque AcostaYKiriakidouM

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In these cases, even a B12-rich diet might not be enough to maintain optimal levels, making it important to explore other ways to replenish your supply