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Gene expression levels were standardized using the z score method, and cluster analysis of DEGs in key pathways was performed using the pheatmap R package [33] based on standardized FPKM values
Cagrilintide directly activates brain satiety centers via amylin pathways, while semaglutide works by slowing gastric emptying and increasing insulin secretion
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However, the disulfide bonds can be formed in the mass spectra as a result of the electro spray ionization process (one-electron oxidation of thiol groups leads to the formation of thiyl radicals which rapidly dimerize) 54