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Blood was first diluted 1:1 in PBS, and PBMCs were isolated using SepMate-50 separation tubes (STEMCELL Technologies) according to the manufacturers instructions

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10.1080/00207454.2018.1481064 69 SayinU.OstingS.HagenJ.RuteckiP.SutulaT

To corroborate our IHC data, western blotting experiments for TNF- and IL-1 showed that their expressions were significantly increased after FA injection, which were alleviated by LA supplementation but without significant difference between the two dose groups, shown in Figure 3