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The peptide content was estimated by UV light spectral density at 280 nm using an extinctions coefficient of 1.1 of 0.1% (g/l) solution that was calculated on the basis of the frequency of tryptophan and tyrosine in vertebrate proteins

Glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are the two most important hormones and both are thought to contribute equally to the effect

In brief, the gradient started with 5% buffer B and increased stepwise to 45% over 45 min, followed by a wash-out at 95% buffer B, all at a flowrate of 300 nl min 1

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Fullerton, M

We also appreciate Judith Kimble and Sarah Crittenden for making the GLP-1::V5 CRISPR/Cas9 strain available prior to publication, for the kind gift of sdn-1 Minimos rescue constructs by Hannes Bulow and Dayse S