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Anfossi, G., Russo, I., Bonomo, K

A preliminary report

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The purification of GST fusions from mammalian cells using GSH-coupled magnetic beads usually requires the removal of the competitively binding endogenous GSH ( In brief, we develop a gene transfection-positive cell sorting system utilizing GPI-anchored GST as the affinity tag that enables positive cell isolation using GSH magnetic beads